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[cGAS/STING signaling pathways induces the secretion of type Ⅰ interferon in porcine alveolar macrophages infected with porcine circovirus type 2]

Authors :
Hongbo, Chen
Feng, Li
Wenyan, Lai
Yuhao, Fang
Mingyong, Jiang
Dianning, Duan
Xiaoyan, Yang
Source :
Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 37(9)
Publication Year :
2021

Abstract

In order to study the signal pathway secreting type Ⅰ interferon in porcine alveolar macrophages (PAMs) infected with porcine circovirus type 2 (PCV2), the protein and the mRNA expression levels of cGAS/STING pathways were analyzed by ELISA, Western blotting and quantitative reverse transcriptase PCR in PAMs infected with PCV2. In addition, the roles of cGAS, STING, TBK1 and NF-κB/P65 in the generation of type I interferon (IFN-I) from PAMs were analyzed by using the cGAS and STING specific siRNA, inhibitors BX795 and BAY 11-7082. The results showed that the expression levels of IFN-I increased significantly at 48 h after infection with PCV2 (P0.05), the mRNA expression levels of cGAS increased significantly at 48 h and 72 h after infection (P0.01), the mRNA expression levels of STING increased significantly at 72 h after infection (P0.01), and the mRNA expression levels of TBK1 and IRF3 increased at 48 h after infection (P0.01). The protein expression levels of STING, TBK1 and IRF3 in PAMs infected with PCV2 were increased, the content of NF-κB/p65 was decreased, and the nuclear entry of NF-κB/p65 and IRF3 was promoted. After knocking down cGAS or STING expression by siRNA, the expression level of IFN-I was significantly decreased after PCV2 infection for 48 h (P0.01). BX795 and BAY 11-7082 inhibitors were used to inhibit the expression of IRF3 and NF-κB, the concentration of IFN-I in BX795-treated group was significantly reduced than that of the PCV2 group (P0.01), while no significant difference was observed between the BAY 11-7028 group and the PCV2 group. The results showed that PAMs infected with PCV2 induced IFN-I secretion through the cGAS/STING/TBK1/IRF3 signaling pathway.

Details

ISSN :
18722075
Volume :
37
Issue :
9
Database :
OpenAIRE
Journal :
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Accession number :
edsair.pmid..........34990ae82f9c29875dc38bdc067e0864