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HERV-Derived
- Source :
- International Journal of Molecular Sciences
- Publication Year :
- 2021
-
Abstract
- (1) Background: The ERVPb1 gene in humans is derived from an envelope (Env) gene of a human endogenous retrovirus group, HERV-P(b). The ERVPb1 gene reportedly has a conserved open reading frame (ORF) in Old World monkeys. Although its forced expression led to cell-fusion in an ex vivo cell culture system, like other Env-derived genes such as syncytin-1 and -2, its mRNA expression is not placenta-specific, but almost ubiquitous, albeit being quite low in human tissues and organs, implying a distinct role for ERVPb1. (2) Methods: To elucidate the cell lineage(s) in which the ERVPb1 protein is translated in human development, we developed a novel, highly sensitive system for detecting HERV-derived proteins/peptides expressed in the tissue differentiation process of human induced pluripotent stem cells (iPSCs). (3) Results: We first determined that ERVPb1 is also conserved in New World monkeys. Then, we showed that the ERVPb1 protein is translated from a uniquely spliced ERVPb1 transcript in hematopoietic cell lineages, including a subset of macrophages, and further showed that its mRNA expression is upregulated by lipopolysaccharide (LPS) stimulation in primary human monocytes. (4) Conclusions: ERVPb1 is unique to Simiiformes and actually translated in hematopoietic cell lineages, including a subset of macrophages.
- Subjects :
- Gene Editing
organoid generation
retrovirus-derived genes (RVDs)
Genes, Viral
hemangioblast cells
Macrophages
Endogenous Retroviruses
Induced Pluripotent Stem Cells
a simiiforme-specific gene
Cell Differentiation
Haplorhini
Article
Cell Line
macrophages
Viral Proteins
human genome
hematopoietic cells
Animals
Humans
human iPSCs
CRISPR-Cas Systems
Viral Fusion Proteins
an Env-derived ERVPb1 gene
Fluorescent Dyes
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 22
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- International journal of molecular sciences
- Accession number :
- edsair.pmid..........2fbfaab105aad3284f58498a8ca45050