[Efficacy of Quercetin-sensitized Adriamycin for Treatment of Refractory Acute Leukemia]

To investigate the chemotherapeutic efficency of quercetin sensitized adriamycin.CCK-8 was used to detect the inhibitory effect of different doses of adriamycin, quercetin and quercetin combined with adriamycin on the proliferation of primary leukemia cells from patients with clinically refractory acute leukemia. Quercetin, adriamycin and their combination were used to treat non-irradiated T-ALL leukemia mice to observe the changes of survival curve and myocardial injury.There was no significant difference in the inhibition rate of primary leukemia cell proliferation between the adriamycin concentration group (6, 0.6 and 0.06 μg/ml) and the adriamycin half-dose (3, 0.3 and 0.03 μg/ml) plus quercetin (0.25 mmol/L) group at three different time points (24, 48 and 72 hours). There was a significant difference in the inhibition rate of primary leukemia cell proliferation among the drug concentration groups, and the inhibition rate of primary leukemia cell proliferation was time-and concentration-dependent (rQuercetin can inhibit the proliferation of primary leukemia cells in a time-dependent manner. Quercetin combined with adriamycin inhibit the proliferation of primary leukemia cells significantly, and had synergistic and additive effects on the proliferation of primary leukemia cells, and the inhibiting effect of quercetin combined with adriamycin is concentration-and time-dependent. Quercetin combined with high-dose adriamycin can significantly prolong the survival time of non-irradiated T-ALL leukemia mice and reduce the myocardial damage caused by adriamycin.槲皮素增敏阿霉素治疗难治耐药急性白血病疗效的研究.探讨槲皮素增敏阿霉素的化疗效果.应用CCK-8 检测不同剂量阿霉素、槲皮素及槲皮素联合阿霉素对临床难治耐药急性白血病患者的原代白血病细胞增殖的抑制作用；用槲皮素、阿霉素及二者联合用药作用于非照射T-ALL白血病小鼠，观察小鼠生存期的变化，以及对于心肌损伤的程度.在3个不同时间点24、48和72 h，不同阿霉素浓度组（6、0.6和0.06 μg/ml）与阿霉素半剂量组（3、0.3和0.03 μg/ml）+ 槲皮素浓度0.25 mmol/L组两两比较发现，对原代白血病细胞增殖抑制率总体差异无统计学意义；各药物浓度组中原代白血病细胞增殖抑制率组间比较总体差异有统计学意义，对原代白血病细胞增殖抑制作用呈浓度和时间依赖性(r槲皮素对原代白血病细胞有增殖抑制作用，其抑制细胞增殖效应表现时间依赖性；槲皮素联合阿霉素后对原代白血病细胞的增殖抑制作用明显，对原代白血病细胞的增殖抑制具有协同和相加作用，且其抑制细胞增殖效应表现为浓度和时间依赖性。槲皮素联合高剂量阿霉素可显著延长非照射T-ALL白血病小鼠的生存期并降低阿霉素对于心肌的损伤.