Back to Search
Start Over
Methods for the Detection of Circulating Pseudogenes and Their Use as Cancer Biomarkers
- Source :
- Methods in molecular biology (Clifton, N.J.). 2324
- Publication Year :
- 2021
-
Abstract
- Pseudogenes, once considered the "junk remnants of genes," are found to significantly affect the regulatory network of healthy and cancer cells, as well as to be highly specific markers of cancer cell identity. Qualitative and quantitative analysis of pseudogenes has a diagnostic and prognostic value in cancer research via the detection of cell-free pseudogenic DNA circulating throughout the body. Exosomes, nanoparticles with a lipid membrane secreted by almost all types of cells, carry cellular-blueprint molecules, including pseudogenic DNA, as cancer-specific cargo. Therefore, it is vital to develop better laboratory techniques and protocols to identify exosome-associated pseudogenes.
- Subjects :
- Base Sequence
Reverse Transcriptase Polymerase Chain Reaction
DNA, Single-Stranded
DNA
DNA, Neoplasm
Nanog Homeobox Protein
Fetal Blood
Prognosis
Transfection
Culture Media
Mutagenesis, Insertional
Neural Stem Cells
Cell-Derived Microparticles
Culture Media, Conditioned
Neoplasms
Sequence Homology, Nucleic Acid
Biomarkers, Tumor
Tumor Cells, Cultured
Humans
RNA, Messenger
Glioblastoma
Pseudogenes
Endothelial Progenitor Cells
Subjects
Details
- ISSN :
- 19406029
- Volume :
- 2324
- Database :
- OpenAIRE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Accession number :
- edsair.pmid..........2ab60f6a579beb6d4f2777abd50e98b2