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Silibinin down-regulates expression of secreted phospholipase A2 enzymes in cancer cells
- Source :
- Anticancer research. 34(4)
- Publication Year :
- 2014
-
Abstract
- Silibinin, a naturally-occurring flavonoid produced by milk thistle, possesses antioxidant, anti-inflammatory and cancer-preventive activities. In the current study, we examined the effects of silibinin on the expression of secreted phospholipase A2 (sPLA2) enzymes, especially those of group IIA (hGIIA), which play a crucial role in inflammation and carcinogenesis.The effects of silibinin on sPLA2 expressions in human HepG2 hepatoma and PC-3 prostate cancer cells were analyzed using quantitative reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay technique.Silibinin inhibited the expression of hGIIA in unstimulated and cytokine-primed HepG2 and PC-3 cells. The mRNA levels of sPLA2 of groups IB, III and V were also significantly decreased by silibinin. Analyses of transcription factor activation suggest that nuclear factor-κB, but not specificity protein 1 (SP1) is implicated in the silibinin-mediated down-regulation of hGIIA.Silibinin exhibits inhibitory effects on basal and cytokine-induced expression of sPLA2s in cancer cells and therefore, may have the potential to protect against up-regulation of hGIIA and other sPLA2 isoforms during inflammation and cancer.
- Subjects :
- Dose-Response Relationship, Drug
Sp1 Transcription Factor
Down-Regulation
Antineoplastic Agents
Gene Expression Regulation, Neoplastic
Cell Line, Tumor
Neoplasms
Silybin
Neoplastic Stem Cells
RNA Isoforms
Humans
Phospholipases A2, Secretory
Tumor Stem Cell Assay
Cell Proliferation
Signal Transduction
Silymarin
Subjects
Details
- ISSN :
- 17917530
- Volume :
- 34
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Anticancer research
- Accession number :
- edsair.pmid..........253d66774b7c0d2213624147f72b4ae2