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Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting
- Source :
- Methods in molecular biology (Clifton, N.J.). 2236
- Publication Year :
- 2020
-
Abstract
- Flow cytometry and magnetic bead technology enable the separation of cell populations with the highest degree of purity. Here, we describe protocols to sort bovine neutrophils from blood, the labeling and sorting, including gating strategies. We also provide advice to preserve neutrophil viability and detail a protocol to measure phagocytosis and oxidative species production.
Details
- ISSN :
- 19406029
- Volume :
- 2236
- Database :
- OpenAIRE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Accession number :
- edsair.pmid..........22cbb3041317442a044e0638f194d74e