Molecular markers for genetics and plant breeding: the MFLP marker system and its application in narrow-leafed lupin (Lupinus angustifolius)

Since the development of molecular markers to tag genes of agronomic traits of interests, molecular markers have played an increasingly significant role in breeding programs. Molecular markers have been implemented for large-scale marker-assisted selection in the breeding program of many important crops including lupin. So far, more than a dozen molecular markers for disease resistance genes and for other agronomic traits of interest have been developed in lupin. The DNA fingerprinting method, "MFLP" has played a pivotal role in the success of lupin breeding program in Australia. Here, we describe the MFLP technique used in lupin breeding which could be easily transferable to other crop species.