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Evidence for local immunosuppression and demonstration of c-myc amplification in pyothorax-associated lymphoma
- Source :
- Histopathology. 39(2)
- Publication Year :
- 2001
-
Abstract
- Pyothorax-associated lymphoma (PAL) develops in the pleural cavity of patients with a long history of pyothorax. Epstein-Barr virus (EBV) is also involved in PAL, similar to lymphomas in immunodeficient patients. Here we examined T-lymphocyte subsets as well as c-myc and REL gene amplification in PAL tissues.We determined the number and distribution of CD4+ and CD8+ T-lymphocytes, to evaluate T-cells in the host immune reaction in seven cases of PAL. As controls, we also studied 10 cases of extranodal diffuse large B-cell lymphoma (DLBL) and 10 cases of nodal DLBL. Chromosomal imbalances in PAL were determined by using comparative genomic hybridization (CGH) analysis. The mean numbers of CD4+ and CD8+ and their ratio were significantly lower in PAL than in nodal DLBL. CGH analysis of PAL showed amplification of the 8q24 chromosomal region. In addition, c-myc amplification was found in four cases of PAL by Southern blot analysis.Our results suggest that the development of PAL may involve a local immunosuppressive environment and that amplification of c-myc might promote tumour progression, as has been described in the development of Burkitt's lymphoma.
- Subjects :
- CD4-Positive T-Lymphocytes
Chromosome Aberrations
Immunosuppression Therapy
Male
Epstein-Barr Virus Infections
Herpesvirus 4, Human
Lymphoma
CD4-CD8 Ratio
Gene Amplification
Genes, myc
Nucleic Acid Hybridization
DNA
CD8-Positive T-Lymphocytes
Middle Aged
Immunophenotyping
Blotting, Southern
Humans
Female
Lymphocyte Count
Empyema, Pleural
In Situ Hybridization
Aged
Neoplasm Staging
Subjects
Details
- ISSN :
- 03090167
- Volume :
- 39
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Histopathology
- Accession number :
- edsair.pmid..........1f0fc91fccd161af44ef7d38acfbc508