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Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis
- Source :
- Nature cell biology. 23(10)
- Publication Year :
- 2020
-
Abstract
- Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis and cancer invasion. Unlike cargo-specific clathrin-mediated endocytosis, the clathrin- and dynamin-independent endocytic pathway (CLIC-GEEC, CG pathway) is considered a bulk internalization route for the fluid phase, glycosylated membrane proteins and lipids. While the core molecular players of CG-endocytosis have been recently defined, evidence of cargo-specific adaptors or selective uptake of proteins for the pathway are lacking. Here we identify the actin-binding protein Swiprosin-1 (Swip1, EFHD2) as a cargo-specific adaptor for CG-endocytosis. Swip1 couples active Rab21-associated integrins with key components of the CG-endocytic machinery-Arf1, IRSp53 and actin-and is critical for integrin endocytosis. Through this function, Swip1 supports integrin-dependent cancer-cell migration and invasion, and is a negative prognostic marker in breast cancer. Our results demonstrate a previously unknown cargo selectivity for the CG pathway and a role for specific adaptors in recruitment into this endocytic route.
Details
- ISSN :
- 14764679
- Volume :
- 23
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Nature cell biology
- Accession number :
- edsair.pmid..........1cf20b0eee60c9e59cb1ecb306bf1797