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Increase of high fluorescence reticulocytes indicates mobilization of peripheral stem cells in children recovering from aplasia after chemotherapy or bone marrow transplantation
- Source :
- Clinical and laboratory haematology. 24(5)
- Publication Year :
- 2002
-
Abstract
- Enumeration of CD34 + cells is the standard assay procedure for optimization of peripheral blood stem cell harvesting. High fluorescence reticulocytes (HFR) have been shown to signal a rebound in haematopoiesis after chemotherapy. The aim of this study was to evaluate HFR determination, as compared to CD34 + cell counts and CFU-GM, as a potential predictor of PBSC counts after recovery from chemotherapy and/or bone marrow transplantation. Twenty-five paediatric patients undergoing intensive courses of chemotherapy and 9 undergoing bone marrow auto or allografts were investigated. In most of our cases, HFR recovered at the same time or earlier than CD34 + cells. Similarly, the rise in HFR preceeded the CFU-GM peak in most of these cases. In no case did we observe a CFU-GM peak without a rise in HFR%. In our experience, the daily relative HFR increase may be used to predict the optimal time for mobilization of stem cells and was therefore of value clinically to confirm the timing of apheresis.
- Subjects :
- Male
Transplantation Conditioning
Adolescent
Graft Survival
Antigens, CD34
Convalescence
Combined Modality Therapy
Transplantation, Autologous
Fluorescence
Reticulocyte Count
Child, Preschool
Hematologic Neoplasms
Neoplasms
Antineoplastic Combined Chemotherapy Protocols
Humans
Transplantation, Homologous
Female
Child
Bone Marrow Transplantation
Follow-Up Studies
Subjects
Details
- ISSN :
- 01419854
- Volume :
- 24
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Clinical and laboratory haematology
- Accession number :
- edsair.pmid..........13837919e2ad2826c03b649fa417528e