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Leptin inhibits hepatocyte growth factor-induced ductal morphogenesis of bovine mammary epithelial cells
- Source :
- The Japanese journal of veterinary research. 54(4)
- Publication Year :
- 2007
-
Abstract
- We examined the effect of stroma-derived factors, hepatocyte growth factor (HGF) and leptin, on morphological differentiation of bovine mammary epithelial cells (BMEC) in collagen gel three-dimensional culture in vitro. BMEC treated with HGF, but not leptin, formed duct-like organoids. The formation of organoids by HGF was enhanced by treatment with a mixture of insulin, cortisol and prolactin, while BMEC treated with the mixture alone did not produce the organoid. In contrast, the formation of organoids by HGF was dose-dependently inhibited by simultaneous addition of leptin, regardless of the presence or absence of the hormone mixture. These results suggest that stroma-derived factors intricately regulate mammary epithelial morphogenesis.
Details
- ISSN :
- 00471917
- Volume :
- 54
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- The Japanese journal of veterinary research
- Accession number :
- edsair.pmid..........07c0332c7f5d6d8a5dac0042b4a90335