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An anti-inflammatory oligopeptide produced by Entamoeba histolytica down-regulates the expression of pro-inflammatory chemokines

Authors :
Dolores, Utrera-Barillas
Juan R, Velazquez
Antonio, Enciso
Samira Muñoz, Cruz
Guadalupe, Rico
Everardo, Curiel-Quesada
Luis M, Teran
Roberto R, Kretschmer
Source :
Parasite immunology. 25(10)
Publication Year :
2004

Abstract

Axenically grown Entamoeba histolytica produces a pentapeptide (Met-Gln-Cys-Asn-Ser) with anti-inflammatory properties that, among others, inhibits the in vitro and in vivo locomotion of human monocytes, sparing polymorphonuclear leucocytes from this effect [hence the name originally given. Monocyte Locomotion Inhibitory Factor (MLIF)]. A synthetic construct of this peptide displays the same effects as the native material. We now added MLIF to resting and PMA-stimulated cells of a human monocyte cell line and measured the effect upon mRNA and protein expression of pro-inflammatory chemokines (RANTES, IP-10, MIP-1alpha, MIP-1beta, MCP-1, IL-8, I-309 and lymphotactin) and the shared CC receptor repertoire. The constitutive expression of these chemokines and the CC receptors was unaffected, whereas induced expression of MIP-1alpha, MIP-1beta, and I-309, and that of the CCR1 receptor--all involved in monocyte chemotaxis--was significantly inhibited by MLIF. This suggests that the inhibition of monocyte functions by MLIF may not only be exerted directly on these cells, but also--and perhaps foremost--through a conglomerate down-regulation of endogenous pro-inflammatory chemokines.

Details

ISSN :
01419838
Volume :
25
Issue :
10
Database :
OpenAIRE
Journal :
Parasite immunology
Accession number :
edsair.pmid..........061c9e34a2c25121f5a850914d89221a