P95-M Mass Spectrometric Proteomic Profiling of Biological Samples using Sample Preparation On Chip (SPOC)

Proteomic profiling of biological samples using mass spectrometry has been a goal for biomarker discovery and disease research for some time. It remains the current challenge to reliably and reproducibly isolate and to sequence the potential biomarkers found in high-throughput screening efforts by tandem mass spectrometry (MS/MS) to differentiate the important and meaningful biomarkers from ambiguous pattern artifacts. To this end, top-down and bottom-up proteomic workflows have yielded advantages and disadvantages, depending upon implementation into the research field.