Degradacija tiametoksama i metabolita klotianidina u suncokretu: od semena do cveta

Cilj istraživanja je praćenje sudbne tiametoksama i klotianidina u biljkama suncokreta, nakon tretiranja semena ovim neonikotinoidom (preparatom Cruiser 600 FS). U ogledu su korišćeni hibridi suncokreta Oliva i Novak. Praćenje degradacije tiametoksama i metabolita klotianidina, je rađeno u cilju ispitivanja vremena razgradnje i njihovog prisustva u različitim fazama razvoja biljke (2 para pravih listova, 6 listova, zvezda, butonizacija i potpuno cvetanje). Metodom tečne hromatografije sa tandem masenom spektrometrijom (LC-MS/MS) uz upotrebu karbofurana-D3 kao internog standarda, nakon QuEChERS ekstrakcije uzoraka biljke suncokreta, urađeno je određivanje ostataka tiametoksama i klotianidina. Nakon obrade podataka, dobijeni su podaci koji ukazuju da je do faze potpunog cvetanja došlo do razgranje neonikotinoida, odnosno da nije bilo njihove detekcije nakon faze butonizacije. The goal of this study is to monitor the fate of thiamethoxam and clothianidin, in sunflower plants, after treating the seeds with this neonicotinoid (Cruiser 600 FS). Olive and Novak sunflower hybrids were used in the experiment. Monitoring of degradation of thiamethoxam and clothianidin was done in order to examine the time of degradation and their presence in different phases of the plant (2 pairs of true leaves, 6 leaves, stars, budding and full flowering). Thiamethoxam and clothianidin residues were determined by liquid chromatography tandem mass spectrometry (LC-MS/MS) using carbofuran-D3 as an internal standard, after the QuEChERS extraction of sunflower plant samples. Obtained data indicate that the neonicotinoids branched to the stage of complete flowering, ie that they were not detected after the budding phase.