Uticaj kompleksa Ru(II) na moguce puteve apoptoze u K562 celijama leukemije

U cilju pronalaženja adekvatne terapije u lecenju maligniteta kompleksi rutenijuma pokazali su zavidan potencijal. Kompleksi Ru(II) sa N-alkilfenotiazinima, hlorpromazinom, trifluoperazinom i tioridazinom, korišceni su u ispitivanju mogucih puteva apoptoze u K562 celijama. Ispitivana je spektrofotometrijski ekstracelularna LDH, ekspresija COX-2, t-JNK, p-JNK i b-aktina imunohemijski nakon SDS elektroforeze. Kompleks Ru(II) sa trifluoperazinom u koncentraciji od 10 μM smanjuje ekspresiju t-JNK, inhibira COX-2 oko 42%, znacajno povecava kolicinu ekstracelularne LDH u odnosu na netretirane K562 celije i time potvrdjuje apoptozu ovih celija Ruthenium complexes are of significant interest in the treatment of malignancies. Ru(II) complexes with N-alkylphenothiazines (chlorpromazine, trifluoperazine, and thioridazine) were used in the study of possible apoptosis pathways in K562 cells. Spectrophotometrically extracellular LDH was quantified and immunochemical expression of COX-2, t-JNK, p-JNK and b-actin after SDS electrophoresis was determined. The Ru(II) complex with trifluoperazine at a concentration of 10 μM reduced t-JNK expression, inhibited COX-2 by about 42%, significantly increased the amount of extracellular LDH compared to the untreated K562 cells and thus confirmed apoptosis. Kratki izvodi radova