Molecular analysis of the goat b-lactoglobulin gene: preliminary results

Analysis of the goat beta-lactoglobulin gene evidenced three differences, two in the 7th exon (a G--A transition in position 83 and a 10 nucleotides, 5'-AGGCCCCTCC-3', insertion/deletion rearrangement phenomenon located downstream nucleotide 104) and one in the 6th intron (an A--G transition located 7 nucleotides upstream the 7th exon). In order to identify carriers of the two exonic mutations, a PCR-RFLP method of analysis has been set up. The frequency of the insertion in a local goat population reared in province of Naples and in Girgentana goats reared in Basilicata and Sicilia was 0.09 and 0.01 respectively