HPLC determination of flavone in human serum

In this paper an analytical method for the extraction and the determination by HPLC-UV of flavone (2-phenyl-4-H-1-benzopyran-4-one) in human serum has been developed. This analyte may have some effects on drug metabolism, interacting with microsomial hepatic enzymes. The proposed method is rapid and simple, with high recoveries (98%), good sensitivity (LOD 7,5 g/mL) and reproducibility (C.V. 8%) and could be utilized for routine epidemiological analysis. Preliminary pharmacokinetics studies showed that flavone hits the maximum blood level into two hours.