PLoS Neglected Tropical Diseases

p. 1-10 Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-01-08T13:08:22Z No. of bitstreams: 1 journal.pntd.0000084.pdf: 1019025 bytes, checksum: 71900033d3c58346ec845b6c7211cb0a (MD5) Approved for entry into archive by Flávia Ferreira (flaviaccf@yahoo.com.br) on 2014-01-10T16:33:53Z (GMT) No. of bitstreams: 1 journal.pntd.0000084.pdf: 1019025 bytes, checksum: 71900033d3c58346ec845b6c7211cb0a (MD5) Made available in DSpace on 2014-01-10T16:33:53Z (GMT). No. of bitstreams: 1 journal.pntd.0000084.pdf: 1019025 bytes, checksum: 71900033d3c58346ec845b6c7211cb0a (MD5) Previous issue date: 2007 Background: Sand fly saliva has an array of pharmacological and immunomodulatory components, and immunity to saliva protects against Leishmania infection. In the present study, we have studied the immune response against Lutzomyia intermedia saliva, the main vector of Leishmania braziliensis in Brazil, and the effects of saliva pre-exposure on L. braziliensis infection employing an intradermal experimental model. Methodology/principal findings: BALB/c mice immunized with L. intermedia salivary gland sonicate (SGS) developed a saliva-specific antibody response and a cellular immune response with presence of both IFN-γ and IL-4. The inflammatory infiltrate observed in SGS-immunized mice was comprised of numerous polymorphonuclear and few mononuclear cells. Mice challenged with live L. braziliensis in the presence of saliva were not protected although lesion development was delayed. The inoculation site and draining lymph node showed continuous parasite replication and low IFN-γ to IL-4 ratio, indicating that pre-exposure to L. intermedia saliva leads to modulation of the immune response. Furthermore, in an endemic area of cutaneous leishmaniasis, patients with active lesions displayed higher levels of anti-L. intermedia saliva antibodies when compared to individuals with a positive skin test result for Leishmania. Conclusion: These results show that pre-exposure to sand fly saliva plays an important role in the outcome of cutaneous leishmaniasis, in both mice and humans. They emphasize possible hurdles in the development of vaccines based on sand fly saliva and the need to identify and select the individual salivary candidates instead of using whole salivary mixture that may favor a non-protective response.