Obten????o de formas farmac??uticas t??picas para o tratamento de vulvovaginites, contendo extrato seco de Endopleura uchi (Huber) Cuatrecasas e ??leo de Bertholletia excelsa H. B. K

Submitted by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2019-12-03T15:47:53Z No. of bitstreams: 2 Reprodu????o N??o Autorizada.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2019-12-03T18:47:09Z (GMT) No. of bitstreams: 2 Reprodu????o N??o Autorizada.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Made available in DSpace on 2019-12-03T18:47:09Z (GMT). No. of bitstreams: 2 Reprodu????o N??o Autorizada.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2019-09-10 92-994719005 Two very important plants of the Brazilian Amazon, Bertholletia excelsa H. B. K. (B. excelsa) and Endopleura uchi (Huber) Cuatrec. (E. uchi), have aroused the interest of the scientific community for the development of phytotherapics and phytocosmetics, due to the functional potential and wide use by traditional medicine. The aim of this research was to obtain two topical dosage forms containing dry extract of E. uchi and B. excelsa oil, aiming at the treatment and prevention of vulvovaginitis. In the characterization and quality control of plant raw materials, both presented indices adequate to those recommended by Brazilian legislation. At the stage of the technological studies, a factorial design was carried out to optimize and standardize the aqueous extraction solution (SE) of E. uchi, whose results demonstrated by determining the content of total polyphenols, total solids and bergenin, that the best extraction method was performed with 10% drug: solvent ratio (m/v) under decoction for 5 minutes. In order to verify possible technological differences (yield and humidity) of the dry extract (ES), without the addition of adjuvants, the SE drying was performed in two different equipments, the fluidized bed drying and the spray dryer. The most appropriate spouted bed, with an operating yield of 66.73% and an ES humidity of 4.16 g%. The ES showed activity against C. albicans (ATCC 90028), C. Krusei (ATCC 6258), C. parapsilosis (ATCC 90018) and C. tropicalis (ATCC 750). Nine liquid soap formulations and three cream formulations with different concentrations of ES E. uchi and B. excelsea oil were evaluated. In both cases only one formulation showed technological feasibility and both containing 2% ES and 2% oil. Analysis of antifungal activity showed that the formulations were most effective against C. krusei (liquid soap = 25 ??g/mL IC50 and 50 ??g/mL IC50 cream) and C. parapsilosis (25 ??g/mL IC50 = liquid soap and 25 ??g/ml IC50 cream). Both formulations were analyzed for 30 days for stability when stored at different temperatures (5 ??C, 25 ??C and 40 ??C), including the icethaw cycle. The study revealed that the cream formulation was stable throughout the storage period, under all conditions tested, on the other hand, the liquid soap formulation showed some instabilities, indicating the need for more rigor and careful storage. Duas plantas muito importantes da Amaz??nia Brasileira, Bertholletia excelsa H. B. K. (B. excelsa) e Endopleura uchi (Huber) Cuatrec. (E. uchi), t??m despertado o interesse da comunidade cient??fica para o desenvolvimento de fitoter??picos e fitocosm??ticos, devido ao potencial funcional e amplo uso pela medicina tradicional. O objetivo desta pesquisa foi a obten????o de duas formas farmac??uticas para uso t??pico contendo extrato seco de E. uchi e ??leo de B. excelsa, visando o tratamento e a preven????o de vulvovaginites. Na caracteriza????o e controle de qualidade das mat??rias-primas vegetais, ambas apresentaram ??ndices adequados aos preconizados pela legisla????o brasileira. Na etapa dos estudos tecnol??gicos foi realizado um planejamento fatorial para otimiza????o e padroniza????o da solu????o extrativa (SE) aquosa de E. uchi, cujos resultados demonstraram atrav??s da determina????o do teor de polifenois totais, s??lidos totais e de bergenina, que o melhor m??todo extrativo foi o realizado com 10% de rela????o droga:solvente (m/V) sob decoc????o por 5 minutos. A fim de verificar poss??veis diferen??as tecnol??gicas (rendimento e umidade) do extrato seco (ES), sem adi????o de adjuvantes, a secagem da SE foi realizada em dois equipamentos distintos, o leito de jorro (fluidized bed drying) e o spray dryer, sendo o leito de jorro mais apropriado, com rendimento operacional de 66,73% e umidade do ES de 4,16 g%. O ES apresentou atividade contra C. albicans (ATCC 90028), C. Krusei (ATCC 6258), C. parapsilosis (ATCC 90018) e C. tropicalis (ATCC 750). Foram avaliadas 9 formula????es de sabonete l??quido e 3 formula????es de creme com diferentes concentra????o de ES de E. uchi e ??leo de B. excelsea. Em ambos os casos apenas uma formula????o apresentou viabilidade tecnol??gica e, ambas contendo 2% de ES e 2% de ??leo. A an??lise da atividade antif??ngica mostrou que as formula????es foram mais eficazes contra C. krusei (sabonete l??quido = CI50 de 25 ??g/mL e creme CI50 de 50 ??g/mL) e C. parapsilosis (sabonete l??quido = CI50 de 25 ??g/mL e creme CI50 de 25 ??g/mL). Ambas as formula????es foram analisados durante 30 dias para verificar a estabilidade quando armazenadas em diferentes temperaturas (5 oC; 25 oC e 40 oC), incluindo o ciclo de gelo-degelo. O estudo revelou que a formula????o em creme se mostrou est??vel durante todo o per??odo de armazenamento, em todas as condi????es testadas, por outro lado, a formula????o em sabonete l??quido demonstrou algumas instabilidades, indicando a necessidade de maior rigor e cuidados quanto ao seu armazenamento.