Método eficiente, baseado em leitores de microplaca, para detecção de resistência a fungicidas triazóis (IDM) e estrobirulinas (IQe) em populações do patógeno da brusone do trigo

Made available in DSpace on 2019-10-06T16:53:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-07-01. Added 1 bitstream(s) on 2021-07-15T14:35:49Z : No. of bitstreams: 1 S0100-54052018000300236.pdf: 1896292 bytes, checksum: d977f317fadb65f08074881c31125eca (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) This study proposes the application of a method based on an automated microplate reader to detect resistance to triazole (DMI) and strobilurin (QoI) fungicides in populations of the plant pathogenic hemibiotrophic fungus Pyricularia graminis-tritici (Pygt) from wheat. Availability of an accurate, more rapid and efficient method to detect eventual reduction in sensitivity to fungicides could contribute to facilitate the decision making on chemical management of wheat blast in the country. There are reports of reduction in the sensitivity of triazoles to control wheat blast and evidence of widespread reduction in the sensitivity to strobilurins in populations of the pathogen in Brazil. The microplate method was accurate in discriminating the phenotypic variation in sensitivity among isolates of the pathogen to the DMI fungicides tebuconazole and epoxiconazole and to the QoI fungicide azoxystrobin. Economically, both methods represent high cost, therefore, the efficiency of these methods should be compared. The microplate method was more efficient in the use of resources, with a total operating cost 33% inferior to that of the Petri dish method. Together, the factors accuracy, celerity and efficiency in the use of resources indicated that the microplate method can be used to monitor resistance to fungicides among populations of the wheat blast fungus in the Brazilian agroecosystem. Universidade Estadual Paulista – UNESP, Campus de Ilha Solteira Empresa Brasileira de Pesquisa Agropecuária – EMBRAPA Trigo Universidade Estadual Paulista – UNESP, Campus de Ilha Solteira FAPESP: 2013/10655-4 e 2015/10453-8 CAPES: 2014/25904-2 FAPESP: 2014/25904-2 FAPESP: 2017/03403-0 CNPq: Pq-2 307361/2012-8 e 307295/2015-0 FAPESP: SEG 02.11.04.006.00.00