Parasite Immunology

Acesso restrito: Texto completo. p. 132-136. Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-03-21T18:44:28Z No. of bitstreams: 1 __ac.els-cdn.com_S026441...f5b4f5fe3819c7cce114160d874ad.pdf: 274617 bytes, checksum: 8ae7e8c59e8bd7ef37cfa311f61ad07b (MD5) Made available in DSpace on 2012-03-21T18:44:28Z (GMT). No. of bitstreams: 1 __ac.els-cdn.com_S026441...f5b4f5fe3819c7cce114160d874ad.pdf: 274617 bytes, checksum: 8ae7e8c59e8bd7ef37cfa311f61ad07b (MD5) Previous issue date: 2011 Cutaneous leishmaniasis (CL) is characterized by high production of pro-inflammatory cytokines and development of pathology. Individuals with subclinical L. braziliensis infection (SC) have a positive skin test to leishmania, but do not develop disease. We evaluated whether the downregulation of inflammatory response in SC is mediated by IL-10 and IL-27 and whether IL-17 is associated with control of infection. Participants include SC individuals, patients with CL and healthy subjects. Cytokines protein and mRNA were detected by ELISA and real-time PCR. IFN-c and TNF-a levels were higher in CL than in SC group. The IL-10 levels and mRNA for IL-10 were similar in both SC and CL. mRNA for IL-27 was increased in cells from SC after stimulation with L. braziliensis antigen. There was a tendency for increased levels of IL-17 in SC compared to CL. The weak type 1 immune response observed in SC L. braziliensis infection is not because of the regulatory effects of IL-10 and IL-27. The control of Leishmania infection may be mediated by innate immune response with participation of IL-17. The results from this pilot study warrant further larger studies to investigate the potential contributions of IL-17 and IL-27 to the control of L. braziliensis infection