Non-ionizing radiation effect on the markup of blood cells with 99 mtc in vitro

The energy density of low power, laser and electromagnetic field , has been used in recent years quite often in the medical field . The study of labeling red blood cells with technetium 99m is used for various evaluations in nuclear medicine including the study of blood volume in neurological intensive care . The aim was to evaluate the in fluence of non - ionizing radiation on the red blood cells labeled in vitro . B lood samples from rats of the Wistar strain divided into two groups were used. The first group used the EDTA and heparin as anticoagulants and received low - power laser induction ( L PL) in the following separately energy density : 3, 6, 9 and 18J/cm². The second group received an electromagnetic field (EMF ) of 60 Hz for 2, 4 , 17 and 21 hours separately . After the induction of the laser and the electromagnetic field , the blood samples w ere labeled with technetium - 99m ( 99mTc). The results indicate that the presence of anticoagulants is able to modify the uptake of technetium - 99m by red blood cells . The laser induced decreases the binding capacity of the technetium - 99m from 3J/cm². From th e foregoing it was found that the radiation is non - ionizing EMF with LBP or alters the binding capacity of 99mTc the erythrocytes and the use of heparin as an anticoagulant gave also a higher labeling efficiency compared with the use of EDTA.The non - ionizi ng radiation has a promising power applications of biomedical sciences can be used for marking targets macromolecules in the body in order to diagnostic procedures and disease control.