HLA polymorphism and humoral immune response to HLA antigens in renal transplant candidates from North/Northwestern of Parana State

The HLA systems (Human Leukocyte Antigen) have a prominent role among the biological systems involved in the rejection process. In this study, we evaluated the HLA class I (HLAA,-B and-C) and class II (HLA-DRB1, -DQA1 and -DQB1) allele frequencies and humoral immune response to HLA antigens in chronic renal patients, renal transplant candidates, from Northern/Northwestern of Parana State. HLA typing was performed by the method of polymerase chain reaction-sequence specific primers (PCR-SSO) associated with Luminex technology, using genomic DNA extracted from peripheral blood leukocytes. The evaluation of the presence of anti-HLA antibodies against a panel of HLA antigens (Panel-Reactive Antibodies - PRA) was performed by complement dependent cytotoxicity (CDC), CDC with the addition of anti-human globulin (CDC-AHG), CDC with the addition of dithiothreitol (CDC-DTT) and solid-phase immunoassay associated with Luminex technology (SPI; LS1PRA kit, One Lambda, Inc.), using serum samples. Determination the specificities of anti-HLA antibodies were performed by kits LS1PRA and LS2PRA (SPI; One Lambda, Inc.). The performance of CDC, CDC-AHG, CDC-DTT and SPI methods for detection of anti-HLA antibodies class I was analyzed in 70 serum samples from chronic renal patients. Mean PRA detected by SPI (37.5 ± 34.2%) was higher than the values detected by the other methods. Comparative analyses revealed significant difference between CDC and CDC-AHG (P