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Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis
- Source :
- Brazilian Journal of Microbiology v.37 n.4 2006, Brazilian Journal of Microbiology, Sociedade Brasileira de Microbiologia (SBM), instacron:SBM
- Publication Year :
- 2006
- Publisher :
- Sociedade Brasileira de Microbiologia, 2006.
-
Abstract
- The restriction endonuclease BliAI, an isoschizomer of ClaI, which recognizes the sequence 5'- AT¯CGAT - 3', was purified from a natural isolate identified as Bacillus licheniformis. The restriction endonuclease was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. The restriction endonuclease is active at 37ºC and over a wide range of pH and salt concentration. The molecular weight of the purified restriction enzyme is consistent with a value of 39 kDa.
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Brazilian Journal of Microbiology v.37 n.4 2006, Brazilian Journal of Microbiology, Sociedade Brasileira de Microbiologia (SBM), instacron:SBM
- Accession number :
- edsair.od......3056..2aff81f21c5c6b6082e3e1838f2a30ac