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Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis

Authors :
Chies,Jocelei Maria
Dias,Ana Christina de Oliveira
Maia,Hélio Mauro Moreira
Braga,Luis Paulo da Silva
Astolfi-Filho,Spartaco
Source :
Brazilian Journal of Microbiology v.37 n.4 2006, Brazilian Journal of Microbiology, Sociedade Brasileira de Microbiologia (SBM), instacron:SBM
Publication Year :
2006
Publisher :
Sociedade Brasileira de Microbiologia, 2006.

Abstract

The restriction endonuclease BliAI, an isoschizomer of ClaI, which recognizes the sequence 5'- AT¯CGAT - 3', was purified from a natural isolate identified as Bacillus licheniformis. The restriction endonuclease was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. The restriction endonuclease is active at 37ºC and over a wide range of pH and salt concentration. The molecular weight of the purified restriction enzyme is consistent with a value of 39 kDa.

Details

Language :
English
Database :
OpenAIRE
Journal :
Brazilian Journal of Microbiology v.37 n.4 2006, Brazilian Journal of Microbiology, Sociedade Brasileira de Microbiologia (SBM), instacron:SBM
Accession number :
edsair.od......3056..2aff81f21c5c6b6082e3e1838f2a30ac