Good practices applied to the collection of bovine semen influence in the sperm parameters

Submitted by Michele Mologni (mologni@unoeste.br) on 2022-04-20T13:02:38Z No. of bitstreams: 1 Fernanda Nobre Bandeira Monteiro.pdf: 807991 bytes, checksum: be7359c9421cbe71fbb21c9d95c02945 (MD5) Made available in DSpace on 2022-04-20T13:02:38Z (GMT). No. of bitstreams: 1 Fernanda Nobre Bandeira Monteiro.pdf: 807991 bytes, checksum: be7359c9421cbe71fbb21c9d95c02945 (MD5) Previous issue date: 2021-03-12 One of the limiting factors for the quality of bovine semen is bacterial contamination, which is usually controlled by adding antibiotic supplements to the diluents. The present work evaluated the influence of semen microbial load on quality parameters in the spermogram. Four sets of fresh semen from 25 bulls, donated by an artificial insemination plant, were diluted and plated superficially on 5% defibrinated sheep blood agar, Baird-Parker agar and MacConkey agar. At the same time, the freezing equipment was tested for microbiological contamination. The urease production score in urea broth, fructose fermentation in phenol red broth were evaluated for the isolated microorganisms. Bacterial counts were statistically correlated (p