Influence of the OsAAP1 and OsAAP18 amino acid transporters on the crude protein acumulation and N remobilization in rice plants

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2019-07-18T16:42:05Z No. of bitstreams: 1 2015 - Cassia Pereira Coelho.pdf: 4393249 bytes, checksum: fb85cca04b6e74dd8ba752222181b30d (MD5) Made available in DSpace on 2019-07-18T16:42:05Z (GMT). No. of bitstreams: 1 2015 - Cassia Pereira Coelho.pdf: 4393249 bytes, checksum: fb85cca04b6e74dd8ba752222181b30d (MD5) Previous issue date: 2015-02-12 CAPES FAPERJ CNPq The main goal of the study was to elucidate the functions of the OsAAP1 and OsAAP18 genes and check its influence on the N remobilization to grains filling and storage of proteins in rice plants. To this, were performed experiments to: (1) Phenotypic characterization of T-DNAi nsertional mutant plants of Dong Jin rice varietyand (2) check affinity of transport for amino acids, through functional complementation of yeast mutant strains 22 8AA, unable to transport efficiently L-proline, L-citrulline, L-aspartate and L.glutamate, 22?6AAL, unable to transport efficiently L-lysine and JA248, unable to transport efficiently L-glutamine. The phenotypic analyze of the mutant plants was developed through a greenhouse experiment using theT-DNAs insertonal mutants lines 3A-00581, mutant for the gene LOC_Os07g04180 (OsAAP1), and 4A-00262, mutant for LOC_Os0636210 gene (OsAAP18) and wild type plants.Complementation tests with mutants yeasts were performed on nitrogen-free medium supplemented with L-proline, L-citrulline, L-aspartate, L-glutamate, L-lisyne or L-glutamine as the sole nitrogen source. Frm the results it was concluded that genes OsAAP1 e OsAAP18 encode to proteins involved in nitrogen remobilization to gains in rice plants and OsAAP18 influences rice productivity protein content in grains. O principal objetivo do trabalho foi elucidar a fun??o dos genes OsAAP1 e OsAAP18 e verificar sua influ?ncia sobre a remobiliza??o de N para os gr?os e forma??o de prote?nas de reserva em plantas de arroz. Para isso, foram realizados experimentos para (1) caracteriza??o fenot?pica de plantas de arroz mutantes por inser??o de T-DNA da variedade Dong Jin e (2) verifica??o da afinidade dos transportadores codificados pelos genes OsAAP1 e OsAAP18 por diferentes substratos (amino?cidos) atrav?s de trabalho de complementa??o em leveduras mutantes das cepas 22?8AA, que ? incapaz de transportar eficientemente L-prolina, L-citrulina, L-aspartatoand L-glutamato, 22?6AAL, que ? incapaz de transportar eficientemente L-lisina e JA248, que ? incapaz de transportar eficientemente L-glutamina, carregando a sequ?ncia codantedos genes OsAAP1 e OsAAP18. A an?lise fenot?pica das plantas mutantes foi realizada atrav?s de experimento conduzido em casa de vegeta??o na UFRRJ utilizando-se as linhagens de plantas mutantes por T-DNA para o gene LOC_Os07g04180 (OsAAP1), 3A- 00581, e para o gene LOC_Os0636210 (OsAAP18), 4A-00262 e plantas tipo selvagem como controle. Testes de complementa??o com as leveduras mutantes foram realizados na Washington State University, utilizando-se meio livre de N, suplementado com L-prolina, L-citrullina, L-aspartato, L-glutamato, L-lisina ou L-glutamina como ?nica fonte de N. Ao final do estudo verificou-se que os genes OsAAP1 e OsAAP18 codificam prote?nas de transporte de amino?cidos envolvidas na remobiliza??o de N para os gr?os em plantas de arroz e OsAAP18influ?ncia o metabolismo de plantas de arroz e a produ??o de gr?os.