Validation and Application of an Ageing Technique for Short-finned Squid (IIlex illecebrosus)

A technique has been developed which simplifies the ageing of short-finned squid (IIlex illecebrosus) through microstructural examination of the statotiths. The spatial pattern of growth increments was studied with the use of light and scanning electron microscopy. Daily growth increments in statoliths were validated by employing chemical "time" markers (strontium and tetracycline) and laboratory-reared animals of known age. Increment formation continued through periods of food deprivation and minimal temperature fluctuations.