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Active focus stabilization for fluorescence microscopy by tracking actively generated infrared spots
- Publication Year :
- 2020
- Publisher :
- Cold Spring Harbor Laboratory, 2020.
-
Abstract
- Maintaining focus during time-lapse experiments remains an important challenge in optical microscopy. In this paper we present an automated focusing system for fluorescence microscopy that can be used in time-lapse experiments. We provide software for flexibly operating the autofocus system and a complete alignment guide. The system is evaluated using a time-lapse experiment with temperature variations on Tetraspeck beads.
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.od......2294..53fbdfe80c28efb608c6c9309924c620
- Full Text :
- https://doi.org/10.1101/2020.09.22.308197