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Active focus stabilization for fluorescence microscopy by tracking actively generated infrared spots

Authors :
van den Berg, Birthe
Van den Eynde, Robin
Müller, Marcel
Vandenberg, Wim
Dedecker, Peter
Publication Year :
2020
Publisher :
Cold Spring Harbor Laboratory, 2020.

Abstract

Maintaining focus during time-lapse experiments remains an important challenge in optical microscopy. In this paper we present an automated focusing system for fluorescence microscopy that can be used in time-lapse experiments. We provide software for flexibly operating the autofocus system and a complete alignment guide. The system is evaluated using a time-lapse experiment with temperature variations on Tetraspeck beads.

Details

Language :
English
Database :
OpenAIRE
Accession number :
edsair.od......2294..53fbdfe80c28efb608c6c9309924c620
Full Text :
https://doi.org/10.1101/2020.09.22.308197