Molekulární fylogeneze a genetická diverzita nejbližších příbuzných rodu Pisum

The topic of this work was studied genetic diversity and phylogeny of selected wild species Lathyrus neurolobus Boiss. & Heldr., which is closely related to cultivated pea, using modern methods of molecular phylogenetics. Genotypes of L. neurolobus were obtained from Department of Botany at Palacký University in Olomouc, genomic DNA was isolated of these genotypes and samples were subjected to analysis using SSR and iPBS markers. It was found that for assessment of genetic diversity is suitable method of iPBS markers, where the average value of polymorphic information content is very high (PIC=0,928). According to evaluated markers polymorphism and assembled dendrograms was found that for search of relationship between place of origin and placement in dendrogram, will be appropriate the method of SSR markers, but only in case of selection informative markers. There was also confirmed transferability of SSR markers designed for pea to wild species of another genus.