Using of Flow Analytical Method for Determination of Biological Active Compounds

Disertační práce Abstract 1 Abstract Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Petra Koblová Supervisor: prof. RNDr. Petr Solich, CSc. Title of Doctoral Thesis: Using of flow analytical method for determination of biological active compounds The presented dissertation thesis is dealing with development of HPLC and SIC methods for determination of active substances and degradation products in pharmaceutical formulations. Developed methods were validated and meet all requirements for suitability, precision and reliability. In the first work, HPLC method for determination of active compound clotrimazole and its degradation products (2-chlorophenyl) diphenylmethanol and imidazole in Clotrimazol spray 1 % was developed. Ibuprofen was used as the internal standard. The aim of the next work was to compare separation efficiency particle based and monolithic columns with various lengths. HPLC method for determination of indomethacin and its two degradation products was used for comparison. Ketoprofen was used as an internal standard for data evaluation throughout the study. SIC method for determination of water-insoluble vitamins (retinol acetate, D2, or D3 and tocopherol acetate) was developed. The novel SIC method with the monolithic...