Study of the effect of albendazole in the environment on the antioxidant system of red clover (Trifolium pratense)

Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Gabriela Mikešková Supervisor: prof. Ing. Barbora Szotáková, Ph.D. Title of diploma thesis: Study of the effect of albendazole in the environment on the antioxidant system of red clover (Trifolium pratense) Today, anthelmintics are among the essential drugs for the therapy and prophylaxis of infectious diseases caused by parasitic worms. However, these substances may be further released into the environment by the excrements of treated animals and affect non-target organisms, such as plants, which have the ability to absorb and accumulate various substances. In this study, we focused on the effect of the often used anthelmintic albendazole on the antioxidant system of red clover (Trifolium pratense), which is commonly found in meadows and pastures. The effect of low concentrations of albendazole available in the environment on the antioxidant enzymes catalase, superoxide dismutase, ascorbate peroxidase, peroxidase, glutathione peroxidase, glutathione reductase and glutathione S-transferase was studied. In addition to antioxidant enzymes, we also monitored the concentration of plant photosynthetic pigments chlorophyll a, b and carotenoids. The results were evaluated in control and...