Glutamine uptake in Zymomonas mobilis

Glutamine was transported in Zymomonas mobilis by a mechanism following Michaelis-Menten kinetics with a Km value for glutamine 8 x 10-5 M and a Vmax value of 15.4 nmol.mg-1, min-1 or 40 nmol.mg-1.min-1 for cells growing on complete medium or minimal medium respectively. The transport of glutamine was energy-dependent and more or less specific for glutamine when cell were grown on rich media. Evidence provided via spheroplasts suggests the possible involvement of a periplasmic component in this transport system. © 1990 Kluwer Academic Publishers.