Two-dimensional proteomics as a tool to evaluate nutritional effects in farmed fish

The use of proteomics in fish research is at a relatively early stage compared to terrestrial vertebrates. It has already been applied in the field of fish nutrition, enabling to point out metabolic changes occurring in response to dietary manipulations such as a variation in energy content, dietary nitrogen compostion, lipid composition or the incorporation of plant protein sources in aquafeeds. NR, M de V and TSS acknowledge financial support from Fundação para a Ciência e Tecnologia, Portugal, through grants SFRH/BDP/65578/2009, SFRH/BD/40698/2007 and SFRH/BD/41392/2007 respectively. The experiments presented here were partly funded by Fundação para a Ciência e Tecnologiam through projects PDTC/MAR/105152/2008 (SPECIAL_K), PPCDT/MAR/61623/2004 (SAARGO), PTDC/MAR/716851/2006 (HYDRAA), prize CERATONIA given by Caixa Geral de Depósitos and University of Algarve, and Research Council of Norway through project 165203/S40.