Recolha e investigação de metabólitos secundários de organismos marinhos de duas ilhas açoreanas, Faial e São Jorge

In March and April of 1997 a total of 35 samples of marine organisms were collected from the Azorean Islands of Faial and São Jorge. These samples included 3 species of Chlorophyta, 7 species of Phaeophyta, 10 species of Rhodophyta, 2 species of Chordata, 3 species of Mollusca, and 7 species of Porifera. Of these samples Laxosuberites rugosus (Porifera), and Pachymatisma johnstonia (Porifera), are new records for the Azores. Secondary metabolite investigations of a number of these samples led to the isolation of para-hydroxybenzyl cyanide from the sponge Laxosuberites rugosus, and oxindol from the sponge Tedania anhelans. Both these compounds are reported here from the marine environment for the first time. These compounds were assessed for their human immunodeficiency virus type 1 reverse transcriptase (HIV-1-RT) and tyrosine kinase (TK) inhibition activities, and para-hydroxybenzyl cyanide found to inhibit the activity of TK to 64% at the 200 Fg/ml level. This is the first report of the TK activity of parahydroxybenzyl cyanide.