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G-proteins in skeletal muscle
- Source :
- The Journal of Physiology, The Journal of Physiology, 2010, 588 (Pt 15), pp.2945-60. ⟨10.1113/jphysiol.2010.191593⟩
- Publication Year :
- 2010
- Publisher :
- HAL CCSD, 2010.
-
Abstract
- International audience; A number of G-protein-coupled receptors are expressed in skeletal muscle but their roles in muscle physiology and downstream effector systems remain poorly investigated. Here we explored the functional importance of the G-protein betagamma (Gbetagamma) signalling pathway on voltage-controlled Ca(2+) homeostasis in single isolated adult skeletal muscle fibres. A GFP-tagged Gbeta(1)gamma(2) dimer was expressed in vivo in mice muscle fibres. The GFP fluorescence pattern was consistent with a Gbeta(1)gamma(2) dimer localization in the transverse-tubule membrane. Membrane current and indo-1 fluorescence measurements performed under voltage-clamp conditions reveal a drastic reduction of both L-type Ca(2+) current density and of peak amplitude of the voltage-activated Ca(2+) transient in Gbeta(1)gamma(2)-expressing fibres. These effects were not observed upon expression of Gbeta(2)gamma(2), Gbeta(3)gamma(2) or Gbeta(4)gamma(2). Our data suggest that the G-protein beta(1)gamma(2) dimer may play an important regulatory role in skeletal muscle excitation-contraction coupling.
- Subjects :
- Cav1.1 channel
MESH: Gene Expression
G-protein
MESH: Muscle Contraction
MESH: GTP-Binding Protein beta Subunits
ryanodine receptor
MESH: Animals
[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
skeletal muscle
calcium current
MESH: Mice
MESH: Action Potentials
MESH: Calcium Channels, L-Type
MESH: Muscle Fibers, Skeletal
fungi
excitation-contraction coupling
MESH: Ion Channel Gating
MESH: Dimerization
beta1gamma2 dimer
MESH: Calcium
G-protein coupled receptor
dihydropyridine receptor
calcium channel
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
calcium transient
MESH: Cells, Cultured
Subjects
Details
- Language :
- English
- ISSN :
- 00223751 and 14697793
- Database :
- OpenAIRE
- Journal :
- The Journal of Physiology, The Journal of Physiology, 2010, 588 (Pt 15), pp.2945-60. ⟨10.1113/jphysiol.2010.191593⟩
- Accession number :
- edsair.od......1398..656d817fc3408f5273cd2e718b161b8e