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Improved secretion of native human IGF-1 from gas1 Saccharomyces cerevisiae cells

Improved secretion of native human IGF-1 from gas1 Saccharomyces cerevisiae cells

Authors :
VAI, MARINA
BRAMBILLA, LUCA GIUSEPPE
ORLANDI, IVAN
ALBERGHINA, LILIA
PORRO, DANILO
Rota, N
Ranzi, B
Vai, M
Brambilla, L
Orlandi, I
Rota, N
Ranzi, B
Alberghina, L
Porro, D
Publication Year :
2000
Publisher :
American Society for Microbiology., 2000.

Abstract

We studied the secretion of recombinant human insulin-like growth factor 1 (rhIGF-1) from transformed yeast cells. The hIGF-1 gene was fused to the mating factor α prepro-leader sequence under the control of the constitutive ACT1 promoter. We found that the inactivation of the GAS1 gene in the host strain led to a supersecretory phenotype yielding a considerable increase, from 8 to 55 mg/liter, in rhIGF-1 production.

Details

Language :
English
Database :
OpenAIRE
Accession number :
edsair.od......1299..dd6385e4b6645ea3130c7f5d1e1f8299