Back to Search
Start Over
Improved secretion of native human IGF-1 from gas1 Saccharomyces cerevisiae cells
Improved secretion of native human IGF-1 from gas1 Saccharomyces cerevisiae cells
- Publication Year :
- 2000
- Publisher :
- American Society for Microbiology., 2000.
-
Abstract
- We studied the secretion of recombinant human insulin-like growth factor 1 (rhIGF-1) from transformed yeast cells. The hIGF-1 gene was fused to the mating factor α prepro-leader sequence under the control of the constitutive ACT1 promoter. We found that the inactivation of the GAS1 gene in the host strain led to a supersecretory phenotype yielding a considerable increase, from 8 to 55 mg/liter, in rhIGF-1 production.
- Subjects :
- secretion
GAS1
IGF-1
saccharomyces cerevisiae
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.od......1299..dd6385e4b6645ea3130c7f5d1e1f8299