MEDPOREO (R) STIMULATES OSTEOBLASTS DIFFERENTIATION IN DENTAL PULP DERIVED STEM CELLS

Medpor (R) is an alloplastic material largely employed hi craniofacial reconstruction due to its characteristics of biological compatibility, easy morphological adaptability and mantaining it's initial volume after beeing positioned. To better understand how Medpor (R) promotes bone formation we analyzed the expression levels of bone related genes in stem cells isolated from dental pulp after 15 and 30 days of exposure to the biomaterial. To this aim we focused on the expression levels of bone related genes (RUNX2, SP7, ALPL, SPP1, COL1A1, COL3A1 and FOSL1) and mesenchymal stem cells marker (ENG). Our results suggest that Medpor (R) induces osteoblast phenotype expression in human DPSCs as demonstrated by the activation of osteoblast related genes FOSL1, RUNX2 and COL3A1 and the disappearance of the mesenchymal stem cells marker, ENG.