Most common interferences in immunoassays

The development of technology has enabled fast and easy determination of tumor markers and hormones at all levels of medical and biochemical diagnostics. Markers and hormones are most commonly determined by immunoassays and methods of molecular diagnostics. Immunoassays are based on the specifi c antibody-antigen reaction. Numerous factors during analysis may aff ect the measurement of tumor markers and hormones in patient samples (1). Although immunoassays are sensitive and reproducible they can miss the specifi city and accuracy. Immunoassays are infl uenced by various interferences and most common of them will be explained in text below.
Razvoj tehnologije je omogućio brzo i jednostavno određivanje hormona i markera. Najčešće se određuju imunokemijskim i metodama olekularne biologije. Imunokemijski testovi se temelje na specifi čnoj reakciji protutijelo-antigen. Brojni faktori mogu utjecati na određivanje tumorskih markera i hormona u uzorku krvi. Iako su imunokemijske metode osjetljive i reproducibilne može im nedostajati specifi čnosti i točnosti. Najčešće interferencije su opisane u tekstu.