From 13 to 22 in a second stroke: revisiting the European Eumida sanguinea (Phyllodocidae: Annelida) species complex

Eumida sanguinea is a recognized polychaete species complex which, in previous studies, has been reported to have additional undescribed diversity. We detected nine additional lineages by analysing DNA sequence data (mitochondrial: COI, 16S rRNA and nuclear loci: ITS region and 28S rRNA) of E. sanguinea morphotype populations from a broader sampling effort in European marine waters. Traditional morphological features failed to provide consistent differences or unique characters that could be used to distinguish these Eumida species. However, by complementing DNA data with morphometrics, geographic range, colour and pigmentation patterns, we revealed five new species. Two of these undescribed species derived from the previously signalled Eumida lineages S21 and GB22, which are here named as E. schanderi sp. nov. and E. fenwicki sp. nov., respectively. Three other species are based on newly discovered lineages, namely E. fauchaldi sp. nov., E. langenecki sp. nov. and E. pleijeli sp. nov. From the six new lineages remaining, three are represented by fewer than two well-preserved specimens, which prevented further comprehensive analysis. The last three lineages were only distinct when using mitochondrial markers. Integrative taxonomy is essential to elucidate evolutionary phenomena and eventually allow informed use of species complexes exhibiting stasis in biomonitoring or other ecological studies.
This study was supported by the project NextSea (NORTE-01-0145-FEDER-000032), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). Thanks are due, for the financial support to CESAM (UIDB/50017/2020+UIDP/50017/2020), to FCT/MEC through national funds, and the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020. Marcos A.L. Teixeira was supported by a PhD grant from FCT co-financed by ESF (SFRH/BD/131527/2017). Pedro Vieira work was supported by national funds through the Portuguese Foundation for Science and Technology (FCT, I.P.) in the scope of the project NIS-DNA [PTDC/BIA 29 BMA/29754/2017]. Ascensão Ravara was funded by national funds (OE), through FCT, I.P., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. Financial support to Arne Nygren from the Norwegian Taxonomy Initiative [http://www.biodiversity.no/Pages/135523] (Cryptic polychaete species in Norwegian waters, knr 49-13, pnr 70184228), the Swedish Taxonomy Initiative [https://www.artdatabanken.se/en/the-swedish-taxonomy-initiative/] (Polychaete species complexes in Swedish waters, dnr 140/07 1.4 and 166/08 1.4), and Kungliga Fysiografiska sällskapet Nilsson-Ehle donationerna [https://www.fysiografen.se/sv/].