Back to Search Start Over

Emerging Technologies for Understanding Platelet Diversity

Emerging Technologies for Understanding Platelet Diversity

Source :
Arteriosclerosis Thrombosis and Vascular Biology. 42(5):540-552
Publication Year :
2022
Publisher :
LIPPINCOTT WILLIAMS & WILKINS, 2022.

Abstract

This review discusses our understanding of platelet diversity with implications for the roles of platelets in hemostasis and thrombosis and identifies advanced technologies set to provide new insights. We use the term diversity to capture intrasubject platelet variability that can be intrinsic or governed by the environment and lead to a heterogeneous response pattern of aggregation, clot promotion, and external communication. Using choice examples, we discuss how the use of advanced technologies can provide new insights into the underlying causes of platelet molecular, structural, and functional diversity. As sources of diversity, we discuss the proliferating megakaryocytes with different allele-specific expression patterns, the asymmetrical formation of proplatelets, changes in platelets induced by aging and priming, interplatelet heterogeneity in thrombus organization and stability, and platelet-dependent communications. We provide indications how current knowledge gaps can be addressed using promising technologies, such as next-generation sequencing, proteomic approaches, advanced imaging techniques, multicolor flow and mass cytometry, multifunctional microfluidics assays, and organ-on-a-chip platforms. We then argue how this technology base can aid in characterizing platelet populations and in identifying platelet biomarkers relevant for the treatment of cardiovascular disease.

Details

Language :
English
ISSN :
15244636 and 10795642
Volume :
42
Issue :
5
Database :
OpenAIRE
Journal :
Arteriosclerosis Thrombosis and Vascular Biology
Accession number :
edsair.od........83..790b5f63926db3db82495067ee09b3f0
Full Text :
https://doi.org/10.1161/atvbaha.121.317092