Alteration of Oxidative Stress Biomarkers in Elite Judokas After Supplementation with Molecular Hydrogen

Ispitivanja primene vodonika u kliničkim uslovima su prilično nova, međutim efikasnost molekularnog vodonika u dosadašnjim kliničkim ispitivanjima je evidentna. Cilj ovog istraživanja bio je da se ispita uticaj suplementacije sa vodom obogaćenom molekularnim vodonikom (HRW) na bazalne vrednosti parametara oksidativnog stresa kod vrhunskih džudista. Eksperimentalni tretman odvijao se u dve faze. U prvoj fazi ispitanici eksperimentalne grupe (N=6) su bili suplementirani sa HRW (4g) u trajanju od 28 dana, dok je kontrolna grupa (N=6) koristila placebo. Nakon suplementacije ispitanici su imali „Washout“ period u trajanju od 28 dana, a zatim je ponovljen identičan protokol na ispitanicima u trajanju od 28 dana. U drugoj fazi eksperimentalnog tretmana ispitivani su akutni efekti suplementacije molekularnim vodonikom na acido baznu ravnotežu vrhunskih džudiskinja. U ovoj fazi studije ispitanice eksperimentalne grupe (N=4) su suplementirane sa HRW (4g) , dok je kontrolna grupa (N=4) koristila placebo. Nakon suplementacije ispitanice su imale „Washout“ period u trajanju od 4 dana, a zatim je ponovljen identičan protokol na ispitanicama pri čemu su ispitanice eksperimentalne grupe (N=4) koristile placebo, dok je kontrolna grupa (N=4) suplementirana sa HRW. Rezultati ispitivanja aktivnosti antioksidativnih enzima kod Placebo i HRW grupe ukazuju na pojavu statistički značajnih razlika u antioksidativnom statusu kod vrhunskih džudista. Odgovor organizma usled unosa HRW ogleda se u povećanju aktivnosti enzima SOD i sadržaja GSH usled sprovođenja programirane fizičke aktivnosti. Takođe, trend opadanja sadržaja MDA u HRW grupi je bio detektabilan. Primena HRW nije izazvala statistički značajne promene u biohemijsko hematološkim parametrima. Akutni efekti suplementacije sa HRW uticali su statistički značajno na redukciju akumulacije laktata kao i na povećanje pH krvi nakon primene specifičnog džudo fitnes testa (SJFT) kod vrhunskih džudistkinja. Ipak, primena suplementa nije značajno uticala na poboljšanje performansi džudistkinja. Dobijeni rezultati ukazuju na ergogenu antioksidantnu moć molekularnog vodonika, pri čemu su dalja istraživanja neophodna kako bi se podesili uslovi u kojima bi molekularni vodonik ispoljio svoje maksimalno delovanje i u sportu. Research of hydrogen application are quite new in clinical conditions, however the efficiency of molecular hydrogen in previous clinical studies is evident. The aim of this study was to investigate the effect of supplementation with hydrogen rich water (HRW) on baseline oxidative stress parameters in elite male judokas. Experimental treatment was carried out in two phases. In the first phase, experimental group (N=6) were supplemented daily with HRW (4g) for 28 days, while the control group (N=6) was used placebo. After supplementation intake all subjects had "washout" period for 28 days, after which the same protocol was repeated for a period of 28 days. In the second stage of experimental treatment, acute effects of supplementation with molecular hydrogen on acid base balance of elite female judokas were examined. In this phase of the study, subjects from experimental group (N=4) were supplemented daily with HRW (4g), while the control group (N=4) used placebo. After supplementation all subjects had a "washout" period for a 4 days, after which the same protocol was repeated wherein the members of the experimental group (N=4) used placebo, while the control group (N=4) was supplemented daily with HRW. The results of analyzed antioxidant enzymes activity in placebo and HRW group indicated on the occurrence of statistically significant differences in antioxidant status of elite judo athletes. Obtained results indicate that response of the body due to intake HRW is reflected in increasing levels of SOD and GSH due to the implementation of programmed physical activity. Also, trend of MDA decreasing in HRW group was detectable. In addition, application of HRW did not cause negative changes in the biochemical and hematological parameters. Acute effects of supplementation were affected to a statistically significant reduction in lactate accumulation and pH increase after applied special judo fitness test (SJFT) on the top judoka. However, use of supplements had no significant effect on improving performance in elite judokas. Obtained results confirm ergogenic antioxidant power of molecular hydrogen, wherein further studies are necessary in order to adjust the conditions in which molecular hydrogen will exerted its maximum in sport.