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MsNRAMP2 Enhances Tolerance to Iron Excess Stress in Nicotiana tabacum and MsMYB Binds to Its Promoter

Authors :
Bi, Run-Tian Li
Yun-Jiao Yang
Wen-Jun Liu
Wen-Wei Liang
Miao Zhang
Shi-Chen Dong
Yong-Jun Shu
Dong-Lin Guo
Chang-Hong Guo
Ying-Dong
Source :
International Journal of Molecular Sciences; Volume 24; Issue 14; Pages: 11278
Publication Year :
2023
Publisher :
Multidisciplinary Digital Publishing Institute, 2023.

Abstract

Iron(Fe) is a trace metal element necessary for plant growth, but excess iron is harmful to plants. Natural resistance-associated macrophage proteins (NRAMPs) are important for divalent metal transport in plants. In this study, we isolated the MsNRAMP2 (MN_547960) gene from alfalfa, the perennial legume forage. The expression of MsNRAMP2 is specifically induced by iron excess. Overexpression of MsNRAMP2 conferred transgenic tobacco tolerance to iron excess, while it conferred yeast sensitivity to excess iron. Together with the MsNRAMP2 gene, MsMYB (MN_547959) expression is induced by excess iron. Y1H indicated that the MsMYB protein could bind to the “CTGTTG” cis element of the MsNRAMP2 promoter. The results indicated that MsNRAMP2 has a function in iron transport and its expression might be regulated by MsMYB. The excess iron tolerance ability enhancement of MsNRAMP2 may be involved in iron transport, sequestration, or redistribution.

Details

Language :
English
ISSN :
14220067
Database :
OpenAIRE
Journal :
International Journal of Molecular Sciences; Volume 24; Issue 14; Pages: 11278
Accession number :
edsair.multidiscipl..8c83bc7b79566f50a2af84c046d4eb11
Full Text :
https://doi.org/10.3390/ijms241411278