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PCR-Based Detection and Genetic Characterization of Parainfluenza Virus 5 Detected in Pigs in Korea from 2016 to 2018

Authors :
Chung, Ha-Thai Truong
Van-Giap Nguyen
Le-Bich-Hang Pham
Thi-My-Le Huynh
Jasper Lee
Su-Jin Hwang
Jae-Myun Lee
Hee-Chun
Source :
Veterinary Sciences; Volume 10; Issue 7; Pages: 414
Publication Year :
2023
Publisher :
Multidisciplinary Digital Publishing Institute, 2023.

Abstract

This study applied a molecular-based method to detect parainfluenza virus 5 (PIV5) collected from 2016 to 2018 in nine provinces of Republic of Korea. We demonstrated that PIV5 was detectable in both serum and pooled organs at an average positive rate of 1.78% (99/5566). Among these, the complete genome sequence of 15,246 nucleotides was obtained for 12 field strains. Three out of the 12 strains had the lowest genetic identity (96.20–96.68%) among the 21 porcine PIV5 genomes collected in Germany, China, India, and Republic of Korea from 1998 to 2017. By analyzing a large collection of complete genome sequences of the structural protein-coding F and HN genes, this study proposed a classification of PIV5 into two lineages, 1 and 2, and identified that group 2.2.2 within sub-lineage 2.2 was substantially divergent. The evolution of two structural protein-coding genes was largely under purifying selection. A few codons (6/9 for the F gene, 7/8 for the HN gene) had elevated dN/dS values, which were loaded on internal branches and were predicted to be related to beneficial trait(s) of the virus.

Details

Language :
English
ISSN :
23067381
Database :
OpenAIRE
Journal :
Veterinary Sciences; Volume 10; Issue 7; Pages: 414
Accession number :
edsair.multidiscipl..05d5b72a0161c55168c64c31d7d597fb
Full Text :
https://doi.org/10.3390/vetsci10070414