Effects of Maharishi Amirit Kalash 4 and 5 on non-specific immune functions in mice

The present study was carried out to examine the effects of Maharishi Amrit Kalash 4 (MAK-4) and 5 (MAK-5) on non-specific immunological mechanisms in mice. Mice aged 4 weeks were divided into 3 groups : a no treatment group (control), MAK-4 treated group (MAK-4 group) and MAK-5 treated group (MAK-5 group). The MAK-4 and MAK-5 were given p.o. at a dose of 50 mg/kg per day for 8 weeks. The following results were obtained : 1. MAK-4 and MAK-5 did not affect the body weight gain and food consumption of the mice. 2. The acid phosphatase (APH) and lactate dehydrogenase (LDH) activities of peritoneal macrophages (Mφ) in the MAK-4 and MAK-5 groups increased significantly compared to the control group. 3. Concanavalin A (Con A) induced cell proliferation in the spleen was high in the MAK-4 and MAK-5 groups. These results suggested that MAK-4 and MAK-5 has a stimulatory effect on Mφ because of the increases in the APH and LDH activities in the peritoneal Mφ of mice. MAK-4 and MAK-5 also intensified the T-cell function represented by Con A-induced splenocyte proliferation.