Cryptosporidium oocyst検出法

The protozoa Cryptosporidium parvum has been identified as one of the agents responsible for numerous outbreaks of diarrheal diseases. Detection of C.parvum oocysts in clinical and environmental samples is the key in the diagnosis of cryptosporidiosis in human and in identifying a water-borne and/or food-borne outbreaks. Therfore, it is very important and necessary to have simples, sensitive and specific methods. However, no such perfect methods are available as yet. In this context, we studies the various methods available by modifying at different aspects and steps using samples collected in Nepal. This study revealed that morphological test using microscopy in combination with specific immunological is valuable for quick screening and the genetic method is effective method for identifying Cryptosporidium species.