Unraveling structural and functional features of secondary transport proteins

Although xylosides are abundant in nature, knowledge about xyloside transporters is scarce. Recently, it has been shown that the xyloside isoprimeverose is the natural substrate of the XyIP protein of Lactobacillus pentosus, a secondary transport protein belonging to the GPH-family of major facilitators. To characterize this transport protein in more detail, a number of hurdles had to be taken, that is, the substrate had to be synthesized, a transport assay had to be developed and the protein had to be incorporated into an artificial membrane system. ... Zie: Chapter 6