Anti-obesity potential of glutinous black rice bran extract: Anti-adipogenesis and lipolysis induction in 3T3-L1 adipocyte model

An ethanolic glutinous black rice bran extract (RBE) was prepared and used in the experiments to determine the phenolic and flavonoid contents, antioxidation using DPPH and TBARS assay, anti-adipogenesis, and lipolysis induction in a 3T3-L1 pre-adipocyte model to discover its anti-obesity potential. The results showed that RBE was composed of phenolics, flavonoids, and anthocyanins and showed antioxidative activities (8.58±0.14 mg Vit C eq/g extract and 6.06±0.49 mg BHT eq/g extract). Interestingly, its anti-obesity potential was demonstrated in the interruption of the adipocyte life cycle, i.e. a significant anti-adipogenesis effect in the reduction of lipid accumulation (50.86±3.26 to 97.06±8.09% of the negative control, and triglyceride content in 3T3-L1 pre-adipocytes (36.89±2.08 to 90.56±0.67% of the negative control), and lipolysis induction (relative glycerol content 117.62±1.44 and 163.72±10.18% of control). Therefore, implementation into obesity therapy requires further research.