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The PIAS-Like Protein Zimp10 Is Essential for Embryonic Viability and Proper Vascular Development
- Source :
- Molecular and Cellular Biology. 28:282-292
- Publication Year :
- 2008
- Publisher :
- Informa UK Limited, 2008.
-
Abstract
- Members of the PIAS (for protein inhibitor of activated STAT) family play critical roles in modulating the activity of a variety of transcriptional regulators. Zimp10, a novel PIAS-like protein, is a transcriptional coregulator and may be involved in the modification of chromatin through interactions with the SWI/SNF chromatin-remodeling complexes. Here, we investigate the biological role of Zimp10 in zimp10-deficient mice. Homozygosity for the Zimp10-targeted allele resulted in developmental arrest at approximately embryonic day 10.5. Analysis of knockout embryos revealed severe defects in the reorganization of the yolk sac vascular plexus. No significant abnormality in hematopoietic potential was observed in zimp10 null mice. Microarray and quantified reverse transcription-PCR analyses showed that the expression of the Fos family member Fra-1, which is involved in extraembryonic vascular development, was reduced in yolk sac tissues of zimp10 null embryos. Using fra-1 promoter/reporter constructs, we further demonstrate the regulatory role of Zimp10 on the transcription of Fra-1. This study provides evidence to demonstrate a crucial role for Zimp10 in vasculogenesis.
- Subjects :
- Microarray
Biology
Mice
Vasculogenesis
Pregnancy
Transcription (biology)
medicine
Animals
Humans
Protein inhibitor of activated STAT
Yolk sac
Molecular Biology
Transcription factor
Cells, Cultured
Oligonucleotide Array Sequence Analysis
Yolk Sac
Mice, Knockout
Homozygote
Gene Expression Regulation, Developmental
Articles
Cell Biology
Embryo, Mammalian
Molecular biology
Embryonic stem cell
Hematopoiesis
Chromatin
medicine.anatomical_structure
Embryo Loss
Female
Proto-Oncogene Proteins c-fos
Transcription Factors
Subjects
Details
- ISSN :
- 10985549
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Biology
- Accession number :
- edsair.doi.dedup.....ffc5c32d4f682afc18185a9feaf077e2