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Opossum colonic mucosa contains uroguanylin and guanylin peptides
- Source :
- American Journal of Physiology-Gastrointestinal and Liver Physiology. 270:G708-G716
- Publication Year :
- 1996
- Publisher :
- American Physiological Society, 1996.
-
Abstract
- Uroguanylin and guanylin are structurally related peptides that activate an intestinal form of membrane guanylate cyclase (GC-C). Guanylin was isolated from the intestine, but uroguanylin was isolated from urine, thus a tissue source for uroguanylin was sought. In these experiments, uroguanylin and guanylin were separated and purified independently from colonic mucosa and urine of opossums. Colonic, urinary, and synthetic forms of uroguanylin had an isoelectric point of approximately 3.0, eluted from C18 reverse-phase high-performance liquid chromatography (RP-HPLC) columns at 8-9% acetonitrile, elicited greater guanosine 3', 5'-cyclic monophosphate (cGMP) responses in T84 cells at pH 5.5 than pH 8, and were not cleaved and inactivated by pretreatment with chymotrypsin. In contrast, colonic, urinary, and synthetic guanylin had an isoelectric point of approximately 6.0, eluted at 15-16% acetonitrile on C18 RP-HPLC columns, stimulated greater cGMP responses in T84 cells at pH 8 than pH 5.5, and were inactivated by chymotrypsin, which hydrolyzed the Phe-Ala or Try-Ala bonds within guanylin. Uroguanylin joins guanylin as an intestinal peptide that may participate in an intrinsic pathway for cGMP-mediated regulation of intestinal salt and water transport. Moreover, uroguanylin and guanylin in urine may be derived from the intestinal mucosa, thus implicating these peptides in an endocrine mechanism linking the intestine with the kidney.
- Subjects :
- Colon
Physiology
Guanylin
Molecular Sequence Data
Guanosine
Peptide
Cell Line
Gastrointestinal Hormones
chemistry.chemical_compound
Intestinal mucosa
Physiology (medical)
Animals
Chymotrypsin
Amino Acid Sequence
Intestinal Mucosa
Natriuretic Peptides
Cyclic GMP
chemistry.chemical_classification
Water transport
Hepatology
biology
Gastroenterology
Opossums
Peptide Fragments
Isoelectric point
chemistry
Biochemistry
biology.protein
Biological Assay
Peptides
hormones, hormone substitutes, and hormone antagonists
Uroguanylin
Subjects
Details
- ISSN :
- 15221547 and 01931857
- Volume :
- 270
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Gastrointestinal and Liver Physiology
- Accession number :
- edsair.doi.dedup.....ffbffe95c924641e19723629c8f4bfe0