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Biomimetic synthesis of chondroitin sulfate-analogue hydrogels for regulating osteogenic and chondrogenic differentiation of bone marrow mesenchymal stem cells
- Source :
- Materials scienceengineering. C, Materials for biological applications. 117
- Publication Year :
- 2019
-
Abstract
- As a typical representative of crucial glycosaminoglycans (GAGs), chondroitin sulfate (CS) with sulfonated polysaccharide in structures extensively exists in the extracellular matrix (ECM) and exhibits peculiar bioactivity on the regulation of cells behaviors and fates (e.g. proliferation and differentiation) in organisms. Nevertheless, some intrinsic disadvantages of natural CS mainly ascribe to the intricate structure and inhomogeneous composition (especially the uncontrollable sulfonate degrees), resulting in overt restrictions on its physiological functions and applications. Although recent bionic synthesis of artificial GAGs analogues at the molecular level have already provides an efficient strategy to reconstruct GAG for regulating the cellular behaviors and fates, it still remains great challenges to rationally design and synthesize GAGs analogues with special composition and structure for precisely mimicking ECM. Simultaneously, the relevant regulation process of GAG analogues on cell fate needs to be further studied as well. Herein, chondroitin sulfate-analogue (CS-analogue) hydrogels with diverse contents of saccharide and sulfonate units in the networks were fabricated through photo-polymerization and then characterized by Fourier transform infrared (FT-IR) spectroscopy, zeta potential and scanning electron microscope (SEM). Additionally, CS-analogue hydrogels with proper mechanical properties exhibited favorable swelling, degradation performance and prominent cytocompatibility. According to cell cultivation results, CS-analogue hydrogel with a certain proportion of saccharide and sulfonate units presented preferable promotion on the adhesion, spreading, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), shedding light on the significance of saccharide and sulfonate units in regulating cell behaviors. Furthermore, BMSCs cultivated with CS-analogue hydrogels under different culture conditions were also systematically investigated, revealing that with the help of cultivation environment CS-analogue hydrogels owned the remarkable capacity of directing either chondrogenic or osteogenic differentiation of BMSCs. Therefore, it is envisioned that versatile CS-analogue hydrogels would have promising application prospects in the biomedical and clinical fields.
- Subjects :
- Materials science
Cell
Bioengineering
Bone Marrow Cells
02 engineering and technology
Cell fate determination
010402 general chemistry
01 natural sciences
Biomaterials
Glycosaminoglycan
Extracellular matrix
chemistry.chemical_compound
Biomimetics
Osteogenesis
Spectroscopy, Fourier Transform Infrared
medicine
Chondroitin
Chondroitin sulfate
Cells, Cultured
Chondroitin Sulfates
Cell Differentiation
Hydrogels
Mesenchymal Stem Cells
021001 nanoscience & nanotechnology
Chondrogenesis
0104 chemical sciences
Cell biology
medicine.anatomical_structure
chemistry
Mechanics of Materials
Self-healing hydrogels
0210 nano-technology
Subjects
Details
- ISSN :
- 18730191
- Volume :
- 117
- Database :
- OpenAIRE
- Journal :
- Materials scienceengineering. C, Materials for biological applications
- Accession number :
- edsair.doi.dedup.....ffbd8291f3829a664c2163cbae706741